HYBOND-P PVDF膜說明書

美國GE試劑（原安瑪西亞）

貨號RPN303F或25006567，品名：HYBOND-P PVDF MEMBRANE  30CM（30 cm × 3 m）

常備貨產品，產品描述如下：

Amersham Hybond™-P

• Hydrophobic polyvinylidene difluoride （PVDF） membrane optimized for use in protein transfers and/or Western blotting applications.
• Higher mechanical strength than unsupported nitrocellulose with a protein binding capacity of 125 µg/cm².
• Chemically stable allowing the use of a range of solvents for rapid destaining.
• 0.45 µm pore size.

操作說明如下：

The higher physical strength of Hybond™-P gives it significant handling advantages over unsupported nitrocellulose and makes it ideal for stripping and reprobing. Hybond™-P must be wetted in 100% methanol then soaked in distilled water before use.

ECL Plus™, detection of b-tubulin in rat brain homogenate using Hybond™-P.